CALINCA—A Novel Pipeline for the Identification of lncRNAs in Podocyte Disease

Diseases of the renal filtration unit—the glomerulus—are most common cause chronic kidney disease. Podocytes are pivotal cell type for function this filter and focal-segmental glomerulosclerosis (FSGS) is a classic example podocytopathy leading to proteinuria glomerular scarring. Currently, no targeted treatment FSGS available. This lack therapeutic strategies explained by limited understanding defects in podocyte biology FSGS. To date, studies field have focused on protein-coding genes their gene products. However, more than 80% all transcripts produced mammalian cells actually non-coding. Here, long non-coding RNAs (lncRNAs) relatively novel class not been systematically studied date. The appropriate tools facilitate lncRNA research scientific community urgently required due row challenges compared classical analysis pipelines optimized coding RNA expression analysis. we present bioinformatic pipeline CALINCA as solution problem. automatically analyzes datasets from murine models quantifies both annotated de novo assembled lncRNAs. In addition, tool provides in-depth information specificity these lncRNAs, well evolutionary conservation human making crucial basis